pearson correlation or spearman’s rank test  (GraphPad Software Inc)

Journal: PLoS ONE

Article Title: Matriptase Deletion Initiates a Sjögren’s Syndrome-Like Disease in Mice

doi: 10.1371/journal.pone.0082852

Figure Lengend Snippet: Electrical potential (EP) of salivary glands was measured 22 weeks post-AAV2 delivery to glands in live AAV2-Cre ( N = 5) and AAV2-LacZ ( N = 3) mice. Membrane potential of pierced ducts of St14 LoxP/LoxP mice ( N = 2) was used as a control for impaired ductal epithelium. (A) Local depletion of matriptase in SMG from AAV2-Cre animals resulted in a significant EP decrease compared with SMG from AAV2-LacZ control mice. The EP of AAV2-Cre mice was similar to that of pierced glands. (B) Correlation between saliva production and EP of salivary glands from AAV2-Cre mice ( N = 3) and AAV2-LacZ mice ( N = 3). Data shown are the mean ± SEM for each group. Statistical significance was determined using unpaired Student’s t test and Pearson’s rank correlation test, respectively. NS, P = 0.1978, ** P = 0.0044, *** P <0.001. (C) Changes in distribution of proteins associated with tight junctions or salivary gland function induced by the loss of matriptase were visualized by immunofluorescent staining of paraffin-embedded SMG tissue samples from St14 – and St14 + control mice 28 to 40 weeks of age ( N = 2 both groups). Representative confocal images are shown. Apical staining of ductal cells for claudin 3 is shown by arrowheads in both St14 + and St14 − animals. An increase in the signal for claudin 3 was detected in the cytoplasm and basal membrane of ductal cells in St14 – mice compared with St14 + mice, as indicated with arrow; original magnification 40X.

Article Snippet: Correlation of SFR and EP was analyzed by Pearson’s rank correlation test (GraphPad Software Inc., La Jolla, CA, USA).

Techniques: Membrane, Control, Staining